abstract：:Trans-sialidases (TS) are GPI-anchored surface enzymes expressed in specific developmental stages of trypanosome parasites like Trypanosoma cruzi, the etiologic agent of Chagas disease, and T. brucei, the causative agent of sleeping sickness. TS catalyzes the transfer of sialic acid residues from host to parasite glyc...

journal_title：Molecular cell

authors： Buschiazzo A,Amaya MF,Cremona ML,Frasch AC,Alzari PM

更新日期：2002-10-01 00:00:00

abstract：:The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: Th...

journal_title：Molecular cell

authors： Chitikila C,Huisinga KL,Irvin JD,Basehoar AD,Pugh BF

更新日期：2002-10-01 00:00:00

abstract：:The Myc oncoprotein represses initiator-dependent transcription through the POZ domain transcription factor Miz-1. We now show that transactivation by Miz-1 is negatively regulated by association with topoisomerase II binding protein (TopBP1); UV irradiation downregulates expression of TopBP1 and releases Miz-1. Miz-1...

journal_title：Molecular cell

authors： Herold S,Wanzel M,Beuger V,Frohme C,Beul D,Hillukkala T,Syvaoja J,Saluz HP,Haenel F,Eilers M

更新日期：2002-09-01 00:00:00

abstract：:RNA polymerases (RNAPs) are core components of the cellular transcriptional machinery. Progress with functional studies of eukaryotic RNAPs has been delayed by the fact that it has not yet been possible to assemble active enzymes from individual subunits. Archaeal RNAPs are directly comparable to eukaryotic RNAPII in ...

journal_title：Molecular cell

authors： Werner F,Weinzierl RO

更新日期：2002-09-01 00:00:00

abstract：:Sterol accumulation in membranes blocks the exit of SCAP from the ER, preventing SREBP cleavage and reducing cholesterol synthesis. Sterols act through SCAP's sterol-sensing domain by an obscure mechanism. Here, we show that addition of cholesterol to ER membranes in vitro causes a conformational change in SCAP, detec...

journal_title：Molecular cell

authors： Brown AJ,Sun L,Feramisco JD,Brown MS,Goldstein JL

更新日期：2002-08-01 00:00:00

abstract：:Nup98 is a component of the nuclear pore that plays its primary role in the export of RNAs. Nup98 is expressed in two forms, derived from alternate mRNA splicing. Both forms are processed into two peptides through autoproteolysis mediated by the C-terminal domain of hNup98. The three-dimensional structure of the C-ter...

journal_title：Molecular cell

authors： Hodel AE,Hodel MR,Griffis ER,Hennig KA,Ratner GA,Xu S,Powers MA

更新日期：2002-08-01 00:00:00

abstract：:Signaling by TGFbeta regulates the expression of hundreds of genes. The rapid repression of c-Myc stands out because of its roles in growth control and cancer. Recent work shows that c-Myc repression by TGFbeta is mediated by the nuclear translocation of a novel, preformed complex composed of Smad3, E2F4 or E2F5, and ...

journal_title：Molecular cell

authors： Kowalik TF

更新日期：2002-07-01 00:00:00

abstract：:It is generally believed that eukaryotic ribosomes first associate with mRNA in the cytoplasm. However, we show with chromosomal immunostaining and in situ hybridization that ribosomal subunits are present at transcription sites of Drosophila salivary gland chromosomes. Immunostaining was carried out with antibodies s...

journal_title：Molecular cell

authors： Brogna S,Sato TA,Rosbash M

更新日期：2002-07-01 00:00:00

abstract：:To determine whether replicational mutagenesis in the yeast genome is influenced by the positions of active origins, a reporter gene was placed in two orientations at multiple locations within a 39,000 bp region of chromosome III possessing two strong origins. The frequency of mutations resulting from misincorporation...

journal_title：Molecular cell

authors： Pavlov YI,Newlon CS,Kunkel TA

更新日期：2002-07-01 00:00:00

abstract：:It has been known for some time that stimulation of heterotrimeric G protein-coupled receptors can cause cytoskeletal reorganization. A recent report in Developmental Cell demonstrates that the activation of a tyrosine kinase, C-terminal Src kinase, by heterotrimeric G protein subunits provides the trigger for Rho-dep...

journal_title：Molecular cell

authors： Luttrell LM

更新日期：2002-06-01 00:00:00

abstract：:Protein kinase B/Akt plays crucial roles in promoting cell survival and mediating insulin responses. The enzyme is stimulated by phosphorylation at two regulatory sites: Thr 309 of the activation segment and Ser 474 of the hydrophobic motif, a conserved feature of many AGC kinases. Analysis of the crystal structures o...

journal_title：Molecular cell

authors： Yang J,Cron P,Thompson V,Good VM,Hess D,Hemmings BA,Barford D

更新日期：2002-06-01 00:00:00

abstract：:Physical and genetic studies verify that the DNA binding domain of protein gpNu1 (which initiates packaging of phage lambda DNA) is a winged helix-turn-helix (w HTH) and that gpNu1 dimers bind sites that are brought close through DNA bending. ...

journal_title：Molecular cell

authors： Campbell A

更新日期：2002-05-01 00:00:00

abstract：:The Chk2 Ser/Thr kinase plays crucial, evolutionarily conserved roles in cellular responses to DNA damage. Identification of two pro-oncogenic mutations within the Chk2 FHA domain has highlighted its importance for Chk2 function in checkpoint activation. The X-ray structure of the Chk2 FHA domain in complex with an in...

journal_title：Molecular cell

authors： Li J,Williams BL,Haire LF,Goldberg M,Wilker E,Durocher D,Yaffe MB,Jackson SP,Smerdon SJ

更新日期：2002-05-01 00:00:00

abstract：:Analysis of nuclear hormone receptor function in cells derived from xeroderma pigmentosum patients reveals novel links between DNA repair and transcription and points to novel mechanisms of regulating transcriptional regulators. ...

journal_title：Molecular cell

authors： Timmers HT

更新日期：2002-04-01 00:00:00

abstract：:Networks of protein interactions coordinate cellular functions. We describe a bimolecular fluorescence complementation (BiFC) assay for determination of the locations of protein interactions in living cells. This approach is based on complementation between two nonfluorescent fragments of the yellow fluorescent protei...

journal_title：Molecular cell

authors： Hu CD,Chinenov Y,Kerppola TK

更新日期：2002-04-01 00:00:00

abstract：:Whereas most kinesins motor along microtubules, KinI kinesins are microtubule depolymerizing machines. Surprisingly, we found that a KinI fragment consisting of only the motor core is capable of ATP-dependent depolymerization. The motor binds along microtubules in all nucleotide states, but in the presence of AMPPNP, ...

journal_title：Molecular cell

authors： Moores CA,Yu M,Guo J,Beraud C,Sakowicz R,Milligan RA

更新日期：2002-04-01 00:00:00

abstract：:The separation of sister chromatids in anaphase depends on the dissociation of cohesin from chromosomes. In vertebrates, some cohesin is removed from chromosomes at the onset of anaphase by proteolytic cleavage. In contrast, the bulk of cohesin is removed from chromosomes already in prophase and prometaphase by an unk...

journal_title：Molecular cell

authors： Sumara I,Vorlaufer E,Stukenberg PT,Kelm O,Redemann N,Nigg EA,Peters JM

更新日期：2002-03-01 00:00:00

abstract：:Negative selection eliminates thymocytes bearing autoreactive T cell receptors (TCR) via an apoptotic mechanism. We have cloned an inhibitor of NF-kappa B, I kappa BNS, which is rapidly expressed upon TCR-triggered but not dexamethasone- or gamma irradiation-stimulated thymocyte death. The predicted protein contains s...

journal_title：Molecular cell

authors： Fiorini E,Schmitz I,Marissen WE,Osborn SL,Touma M,Sasada T,Reche PA,Tibaldi EV,Hussey RE,Kruisbeek AM,Reinherz EL,Clayton LK

更新日期：2002-03-01 00:00:00

abstract：:DNA breakage is intimately associated with meiotic recombination in the fission yeast Schizosaccharomyces pombe. Sites of prominent DNA breakage were found approximately 25 to approximately 200 kb apart in the genomic regions surveyed. We examined in detail a 501 kb region of chromosome I and found six sites, or tight...

journal_title：Molecular cell

authors： Young JA,Schreckhise RW,Steiner WW,Smith GR

更新日期：2002-02-01 00:00:00

abstract：:Epstein-Barr virus (EBV) causes infectious mononucleosis, establishes long-term latent infections, and is associated with a variety of human tumors. The EBV gp42 glycoprotein binds MHC class II molecules, playing a critical role in infection of B lymphocytes. EBV gp42 belongs to the C-type lectin superfamily, with hom...

journal_title：Molecular cell

authors： Mullen MM,Haan KM,Longnecker R,Jardetzky TS

更新日期：2002-02-01 00:00:00

abstract：:In this issue of Molecular Cell, Zhang et al. and Møller et al. independently report studies of the E. coli Hfq protein, revealing significant sequence similarities with human Sm proteins. Their findings suggest that Hfq, and the Sm and Sm-like (Lsm) proteins, may function in stabilizing interactions between RNA molec...

journal_title：Molecular cell

authors： Donahue WF,Jarrell KA

更新日期：2002-01-01 00:00:00

abstract：:We have identified ARAP1 and ARAP2 and examined ARAP1 as a possible link between phosphoinositide-, Arf-, and Rho-mediated cell signaling. ARAP1 contains Arf GAP, Rho GAP, Ankyrin repeat, Ras-associating, and five PH domains. In vitro, ARAP1 had Rho GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent A...

journal_title：Molecular cell

authors： Miura K,Jacques KM,Stauffer S,Kubosaki A,Zhu K,Hirsch DS,Resau J,Zheng Y,Randazzo PA

更新日期：2002-01-01 00:00:00

abstract：:Cellular DNA damage causes stabilization and activation of the tumor suppressor and transcription factor p53, in part by promoting multiple covalent modifications of the p53 protein, including acetylation. We investigated the importance of acetylation in p53 function and the mechanism by which acetylation influences p...

journal_title：Molecular cell

authors： Barlev NA,Liu L,Chehab NH,Mansfield K,Harris KG,Halazonetis TD,Berger SL

更新日期：2001-12-01 00:00:00

abstract：:Deletion of elongation factor-like 1 (Efl1p), a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2, results in nucle(ol)ar pre-rRNA processing and pre-60S subunits export defects. Efl1p interacts genetically with Tif6p, a nucle(ol)ar protein stably associated with pre-60S subunits and required for t...

journal_title：Molecular cell

authors： Senger B,Lafontaine DL,Graindorge JS,Gadal O,Camasses A,Sanni A,Garnier JM,Breitenbach M,Hurt E,Fasiolo F

更新日期：2001-12-01 00:00:00

abstract：:Proteins that recognize specific DNA sequences play a central role in the regulation of transcription. The tremendous increase in structural information on protein-DNA complexes has uncovered a remarkable structural diversity in DNA binding folds, while at the same time revealing common themes in binding to target sit...

journal_title：Molecular cell

authors： Garvie CW,Wolberger C

更新日期：2001-11-01 00:00:00

abstract：:Colicins kill E. coli by a process that involves binding to a surface receptor, entering the cell, and, finally, intoxicating it. The lethal action of colicin E3 is a specific cleavage in the ribosomal decoding A site. The crystal structure of colicin E3, reported here in a binary complex with its immunity protein (IP...

journal_title：Molecular cell

authors： Soelaiman S,Jakes K,Wu N,Li C,Shoham M

更新日期：2001-11-01 00:00:00

abstract：:FMOC-L-Leucine (F-L-Leu) is a chemically distinct PPARgamma ligand. Two molecules of F-L-Leu bind to the ligand binding domain of a single PPARgamma molecule, making its mode of receptor interaction distinct from that of other nuclear receptor ligands. F-L-Leu induces a particular allosteric configuration of PPARgamma...

journal_title：Molecular cell

authors： Rocchi S,Picard F,Vamecq J,Gelman L,Potier N,Zeyer D,Dubuquoy L,Bac P,Champy MF,Plunket KD,Leesnitzer LM,Blanchard SG,Desreumaux P,Moras D,Renaud JP,Auwerx J

更新日期：2001-10-01 00:00:00

abstract：:Hydroxyl radical footprinting and directed probing from Fe(II)-derivatized IF3 have been used to map the interaction of IF3 relative to 16S rRNA and tRNA(Met)(f) in the 30S ribosomal subunit. Our results place the two domains of IF3 on opposite sides of the initiator tRNA, with the C domain at the platform interface a...

journal_title：Molecular cell

authors： Dallas A,Noller HF

更新日期：2001-10-01 00:00:00

abstract：:The translocon is the gateway to the endoplasmic reticulum (ER). In yeast this is the Sec61p complex. However, new evidence suggests that a second translocon containing the Sec61p homolog Ssh1p provides important flexibility to the ER translocation machinery. ...

journal_title：Molecular cell

authors： Robb A,Brown JD

更新日期：2001-09-01 00:00:00

abstract：:The transcriptional coactivators CBP and p300 are critical regulators of metazoan gene expression. They associate with many different DNA-bound transcription factors through small, conserved domains. We have identified a compactly folded 46 residue domain in CBP and p300, the IRF-3 binding domain (IBiD), and we have d...

journal_title：Molecular cell

authors： Lin CH,Hare BJ,Wagner G,Harrison SC,Maniatis T,Fraenkel E

更新日期：2001-09-01 00:00:00

abstract：:The role of the protein kinase Akt in cell migration is incompletely understood. Here we show that sphingosine-1-phosphate (S1P)-induced endothelial cell migration requires the Akt-mediated phosphorylation of the G protein-coupled receptor (GPCR) EDG-1. Activated Akt binds to EDG-1 and phosphorylates the third intrace...

journal_title：Molecular cell

authors： Lee MJ,Thangada S,Paik JH,Sapkota GP,Ancellin N,Chae SS,Wu M,Morales-Ruiz M,Sessa WC,Alessi DR,Hla T

更新日期：2001-09-01 00:00:00

abstract：:Steroid hormones have rapid nongenomic effects on cell-signaling pathways, but the receptor mechanisms responsible for this are not understood. We have identified a specific polyproline motif in the amino-terminal domain of conventional progesterone receptor (PR) that mediates direct progestin-dependent interaction of...

journal_title：Molecular cell

authors： Boonyaratanakornkit V,Scott MP,Ribon V,Sherman L,Anderson SM,Maller JL,Miller WT,Edwards DP

更新日期：2001-08-01 00:00:00

abstract：:The heterodimeric nuclear cap binding complex (CBC) binds to 5'-capped polymerase II transcripts. It enhances the efficiency of several mRNA maturation steps and is essential for U snRNA nuclear export in multicellular eukaryotes. The 2A crystal structure of human CBC shows that the large subunit, CBP80, comprises thr...

journal_title：Molecular cell

authors： Mazza C,Ohno M,Segref A,Mattaj IW,Cusack S

更新日期：2001-08-01 00:00:00

abstract：:The tumor suppressor protein, p53, plays a critical role in mediating cellular response to stress signals by regulating genes involved in cell cycle arrest and apoptosis. p53 is believed to be inactive for DNA binding unless its C terminus is modified or structurally altered. We show that unmodified p53 actively binds...

journal_title：Molecular cell

authors： Espinosa JM,Emerson BM

更新日期：2001-07-01 00:00:00

abstract：:Herpes simplex virus (HSV) infection requires binding of the viral envelope glycoprotein D (gD) to cell surface receptors. We report the X-ray structures of a soluble, truncated ectodomain of gD both alone and in complex with the ectodomain of its cellular receptor HveA. Two bound anions suggest possible binding sites...

journal_title：Molecular cell

authors： Carfí A,Willis SH,Whitbeck JC,Krummenacher C,Cohen GH,Eisenberg RJ,Wiley DC

更新日期：2001-07-01 00:00:00

abstract：:The accumulation of unfolded protein in the endoplasmic reticulum (ER) attenuates protein synthesis initiation through phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) at Ser51. Subsequently, transcription of genes encoding adaptive functions including the glucose-regulate...

journal_title：Molecular cell

authors： Scheuner D,Song B,McEwen E,Liu C,Laybutt R,Gillespie P,Saunders T,Bonner-Weir S,Kaufman RJ

更新日期：2001-06-01 00:00:00

abstract：:Cell pattern in the ventral neural tube is organized by Sonic hedgehog (Shh) secreted by floor plate cells. To assay the range of direct Shh action, we developed a general method for blocking transduction of Hedgehog (Hh) signals through ectopic expression of a deleted form of the Hh receptor Patched (Ptc), termed Ptc...

journal_title：Molecular cell

authors： Briscoe J,Chen Y,Jessell TM,Struhl G

更新日期：2001-06-01 00:00:00

abstract：:The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and GSK3beta, forms a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of beta-catenin by the proteasome. Siah-1, the human homolog of Drosophila seven in absentia, is a p53-inducible mediator of cell c...

journal_title：Molecular cell

authors： Liu J,Stevens J,Rote CA,Yost HJ,Hu Y,Neufeld KL,White RL,Matsunami N

更新日期：2001-05-01 00:00:00

abstract：:Tet(O) belongs to a class of ribosomal protection proteins that mediate tetracycline resistance. It is a G protein that shows significant sequence similarity to elongation factor EF-G. Here we present a cryo-electron microscopic reconstruction, at 16 A resolution, of its complex with the E. coli 70S ribosome. Tet(O) w...

journal_title：Molecular cell

authors： Spahn CM,Blaha G,Agrawal RK,Penczek P,Grassucci RA,Trieber CA,Connell SR,Taylor DE,Nierhaus KH,Frank J

更新日期：2001-05-01 00:00:00

abstract：:In response to hyperosmotic environments, most eukaryotic cells activate a specialized mitogen-activated protein (MAP) kinase pathway. In S. cerevisiae, the key protein kinase, Hog1, coordinates the transcriptional induction of a variety of genes devoted to osmoadaptation and general stress protection. Depending on th...

journal_title：Molecular cell

authors： Alepuz PM,Jovanovic A,Reiser V,Ammerer G

更新日期：2001-04-01 00:00:00